3D Cell Culture | (주)나노팬텍-Nanofaentech

Nanofaentech 3D Culture System

1. KEY FEATURE

Ease of cell observation

Although majority of nanofibers currently sold commercially are opaque,

the fiber we have developed is transparent to allow observation of cells visually or under fluorescent microscope.

Outstanding cell adhesion

· Provide 3-dimensional adhesiveness similar to that of human body without affecting

the characteristic activation and differentiation of the cell

· Can be applied to epithelial cells, endothelial cells of blood vessel, fibroblast, cancer cell

and immune cell, etc.

Culturing of more than 2 types of cells

There are diversified types of cells in human body. This technology offers a system capable of culturing more than 2 different types of cells together for growth and differentiation of cells.

2. PRACTICALITY OF TECHNOLOGY

From the perspective of products sold

Although majority of nanofibers currently sold commercially are opaque, Can be developed into products for each of diversified culture plate sizes
(currently, 8-well product is available with schedule to launch Transwell prototype)

From the perspective of product specifications

Maintain uniform PVA nanofiber diameter and aperture (300±50nm)

(PCL nanofiber currently being sold in overseas sigma has irregular diameter)

Product diversity

PVA nanofiber containing laminin peptide customized for diversified cells can be manufactured, and 3-dimensional culturing is possible

Collagen

PVA nanofiber

PCL nanofiber

3. CELL LIFE EXTENSION TECHNOLOGY

Current

Sandwich method of culturing cell by having the cell seeded in collagen-coated culture dish and cultured by placing Matrigel on top of the cell

Special culture medium, dexamethasone and insulin, etc. must be included
Even if the hepatocyte survives, its original physiological activation and cell adhesion is substantially degraded
As such, long-term culturing is not possible