Technology of Nanofaentech

Dendritic cell

Macrophage

Hepatocyte

Fibroblast

Lung epithelial cell

Endothelial cell

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Product Conceptual diagram

NIH3T3 cells

CT26 cells

Macrophage

3-dimensional culture and image realization of diversified cells in PVA nanofiber

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DIC Imaging

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3D floor arrangement

2D cell culture

3D cell culture

Peptide- PVA 3D cell culture

3-dimensional culture cell imaging of PVA nanofiber

1

PVA nanofiber containing cell-binding specific peptide or ligand

2

Can apply nanofiber materials for 3-dimensional cell culture, which enables cells culturing

under environment that is as close to human body as possible, to diversified cell culture plates

(from 8-well to 96-plate)

3

Key Features

Ease of cell

observation

Although majority of nanofibers currently sold commercially are opaque,

the fiber we have developed is transparent to allow observation of cells visually or under fluorescent microscope.

Outstanding

cell adhesion

· Provide 3-dimensional adhesiveness similar to that of human body

  without affecting the characteristic activation and differentiation of the cell

· Can be applied to epithelial cells, endothelial cells of blood vessel, 

  fibroblast, cancer cell and immune cell, etc.

Culturing of

more than 2 types of cells

There are diversified types of cells in human body. This technology offers a system capable of culturing more than 2 different types of cells together for growth and differentiation of cells.

4

Practicality of technology

From the perspective of products sold

Although majority of nanofibers currently sold commercially are opaque,

Can be developed into products for each of diversified culture plate sizes

(currently, 8-well product is available with schedule to launch Transwell prototype)

From the perspective of product specifications

Maintain uniform PVA nanofiber diameter and aperture (300±50nm)

(PCL nanofiber currently being sold in overseas sigma has irregular diameter)

Product

diversity

PVA nanofiber containing laminin peptide customized for diversified cells can be manufactured, and 3-dimensional culturing is possible

Collagen

PVA nanofiber

PLC nanofiber

5

Technical performance report

Completion of cytotoxicity test analysis of PVA nanofiber using culture cells of mammals (Osong Advanced Medical Industry Promotions Foundation)

Completion of surface FE-SEM analysis of PVA fiber (Korea Polymer Testing & Research Institute)

Maintain consistent diameter of electrically spun PVA nanofiber

PVA nanofiber containing laminin peptide customized for diversified cells can be manufactured,

and 3-dimensional culturing is possible

Maintain diameter of electrically spun PVA nanofiber within aqueous solution

No statistical significance prior to and after treatment with water (1 week)

PVA nanofiber for 8-well plate (1.2cmx1.2cm)

Cytotoxicity test: Maintain cell survival rate of more than 99%

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Results of official certification of porosity

6

Cell life extension technology

Current

Sandwich method of culturing cell by having the cell seeded in collagen-coated culture dish and cultured

by placing Matrigel on top of the cell

As such, long-term

culturing is not possible

Special culture medium, dexamethasone

and insulin, etc.

must be included

Even if the hepatocyte

survives, its original

physiological activation

and cell adhesion

is substantially degraded

Differentiation of technology

Possession of culturing condition technology to maximize and maintain the survival of hepatocyte

7

Cell culture technology

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Conventional technology

Liver Tissue

This technology

(Illustration) Liver cell culturing

8

Technical intellectual property rights